Project Leads: Andre Zylstra (az308) and Bill Jia (bzj20)
For this year’s Biology Project we will be building light-responsive genetic circuits in E.coli!
Once we have settled into the Biomakespace, our first goal will be to implement a system to make the bacteria generate colour changes in their environment in response to light. Then we intend to move on to a genetic construct which will allow the bacterial coony to perform edge detection i.e. only to produce colour changes where the light / dark boundary falls over the colony.
Finally, we hope to be able to extend previously published work by generating a circuit enabling colonies to perform high-pass filtering, resulting in a kind of image sharpening behaviour. We expect computational modelling of the system will play a significant role in making this possible!
To dig into the finer details of the circuits we will be using, have a look at:
- Levskaya et al. (2005) Synthetic biology: Engineering Escherichia coli to see light. Nature, 438(7067),441-442.
- Tabor et al. (2009). A Synthetic Genetic Edge Detection Program. Cell, 137(7), 1272-1281.
- Molecular Biology at Makespace! (11/3/2017)