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Workshop 4: Testing the gene circuit (11 November)

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In our final workshop, we arrive at the final stage of our bacterial photography project based on a study by Tabor et al (2009) [http://doi.org/10.1016/j.cell.2009.04.048]. Based on their protocol, we inoculated LB media containing ampicillin and streptomycin with E. coli cells of the DH5α strain that had been transformed with the pSR33.4r and the pEDL3 […]

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Workshop 3: Plasmid miniprep (4 November 2018)

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This week we continue with extracting plasmids transformed into bacteria that have grown in a liquid culture, using a commercially available kit. Our procedure started with centrifuging the liquid cultures to pellet the bacterial cells. Then, the cells were lysed (broken apart) using an alkaline buffer so that we could obtain the plasmids in the […]