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Workshop 3: Plasmid miniprep (4 November 2018)

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This week we continue with extracting plasmids transformed into bacteria that have grown in a liquid culture, using a commercially available kit. Our procedure started with centrifuging the liquid cultures to pellet the bacterial cells. Then, the cells were lysed (broken apart) using an alkaline buffer so that we could obtain the plasmids in the […]

Projects

Workshop 1: Gibson design and PCR (21 October 2018)

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And this is our first of four biological project workshops planned this term! These workshops will be covering key steps in the process of engineering genetic constructs to enable our E. coli to detect light and draw edges accordingly using quorum sensing, and will aim to give participants a chance to do hands-on work on […]

bacterialphotography

Bacterial Edge Detection Modeling

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As Lent term comes to a close, the members of the computational subteam of the Bioproject have been wrapping up the coding of the model edge detector system. After some struggles with constants and boundary conditions, we finally succeeded in producing a program that simulates the edge detection circuit! The edge detector works by instructing […]

bacterialphotography

First PCR and Gel at Biomakespace

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We rounded off the Michaelmas term with our first practical session at the newly opened Biomakespace! Our aim for the day was to test out the facilities at Biomakespace by running a PCR followed by performing DNA electrophoresis – both very important molecular biology techniques. PCR, short for Polymerase Chain Reaction, is an extremely useful […]

bacterialphotography

Molecular Biology at Makespace!

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Molecular Biology at Makespace! Today was our first piece of practical molecular biology for the CUSBS Bioproject 2017-18! We met at the Makespace, Mill Lane, as part of the Open Technology Week Science Make-a-thon. Our goal was to test our ability to produce the enzyme β-galactosidase/LacZ from plasmid DNA in an in vitro gene expression […]

openscope

OpenScope Field

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OpenScope Field Optimisation Click here for a detailed budget and timeline. Goals – This project centres around developing the OpenScope  microscope field-use, for example in testing blood and water samples. This project is likely to be carried out with some collaboration with the WaterScope team (developing a water quality testing kit for South Asia). – […]

openscope

OpenScope Automation

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OpenScope Automation Click here for a detailed budget and timeline. Progress Goals – This project centres around high-throughput screening of bacterial colonies and immature plants using a microscope mounted on a desktop CNC machine (a large x,y,z-translation system; see below). Shapeoko desktop CNC machine – Ultimately, the goal is to develop a robot that can […]